Ta3 is required for asymmetrical localization of Cep120 to the daughter centriole.

Abstract

<p>Wild type and <i>Ta3</i> mutant primary mouse embryonic fibroblasts (pMEFs) were coimmunostained for Cep120 and γ ˜tubulin (A) or Odf2 and Cep120 (B), together with DAPI (for nuclei). Arrowheads indicate the specific staining at centrioles. The Cep120 signal at the daughter and mother centrioles (DC and MC, respectively) was quantified for 21 randomly chosen cells, using NIH image J. Cep120 signal ratios were then calculated, specifically the DC to MC ratio and the wild type to Ta3 mutant ratio (average + standard deviation) (C). (D) No significant changes in Cep120 levels in <i>Ta3</i> mutant MEFs. Immunoblots show the expression of endogenous Ta3 and Cep120 in wild type and <i>Ta3</i> mutant MEFs, with tubulin as a loading control.</p