Distribution of fluorescently labeled <i>V. cholerae</i> in the infant mouse small intestine.

Abstract

<p>(A) The small intestines of infant mice co-inoculated with VcRed and VcGreen were divided into three equal parts and the central 1 cm segments of the proximal (P), medial (M) and distal (D) parts were used for plating and microscopic analyses. (B) Numbers of CFUs recovered from homogenates of the proximal (P), medial (M) and distal (D) segments. Bars represent the geometric mean. *, p<0.05, and **, p<0.01, based on ANOVA with Tukey's multiple comparison test. (CEG) Confocal micrographs showing VcRed and VcGreen distribution in the proximal (C), medial (E) and distal (G) segments. Tissue sections were counterstained with phalloidin (blue) to visualize the surface of the epithelium. White arrowheads mark clonal microcolonies. Scale bar = 100 µm. (DFH) The distance separating microcolonies from the base of the villi in the proximal (D), medial (F) and distal (H) SI segments was measured using confocal microscopy in tissue cross sections from mice co-inoculated with VcRed and VcGreen. Data represent the mean ±SD from three mice. The number (n) of microcolonies analyzed is indicated to the left of each panel. Black triangles indicate the average length of villi in each region. (I) Average length of intestinal villi (±SD) in the proximal (P), medial (M) and distal (D) segments. ***, p<0.001, based on ANOVA with Bonferroni's multiple comparison test.</p

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