Peroxidase activation of MAK-1 and MAK-2 pathways in cell fusion mutant vegetative hyphal cells.

Abstract

<p>Western blot analyses using Phospho-p44/42 MAPK antibody were performed to evaluate MAK-1 and MAK-2 activation in wild type (WT) and mutant vegetative hyphal cells in response to peroxidase treatments. Quantitative analyses of the Western blots were performed to determine the levels of phosphorylated MAK-1 and MAK-2 in non-stressed and oxidative-stressed samples (wild type, <i>Δham-6</i>, <i>Δham-7</i>, <i>Δham-8</i>, <i>Δham-9</i>, <i>Δham-10</i>, <i>Δamph-1</i>, and <i>Δwhi-2</i>). A) MAK-1 activation in wild type and mutants in response to peroxidase treatment. B) MAK-2 activation in wild type and mutants in response to peroxidase treatment. The levels of MAK-1 and MAK-2 in the non-stressed wild type sample were set as 100% for the quantitative analysis.</p