Sensitivity of LAMP and PCR methods for the detection of <i>O. volvulus</i> using ten-fold serial dilutions of <i>O. volvulus</i> genomic DNA ranging from 0.001–1.0 ng.

Abstract

<p>Detection of LAMP product using turbidity (<b>A</b>) or hydroxy napthol blue (<b>B</b>). PCR amplification of a ∼200 bp product using LAMP primers F3 and B3 was obtained when <i>O. volvulus</i> genomic DNA was used (<b>C</b>). Molecular weight marker (MW) is indicated.</p