Phylogenic tree of insect short neuropeptide F prepropeptide sequences from different insect species.

Abstract

<p>Amino acid sequences were analyzed by MEGA (version 5.05); the phylogenetic tree was constructed using the neighbor-joining method. The number 86 indicates the bootstrap confidence level. Abbreviations on branches correspond to the genus and species name, and these are identical to those described in the legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109590#pone-0109590-g002" target="_blank">Figure 2</a>, with the addition of the Southern house mosquito, <i>Culqu</i> (<i>Culex quinquefasciatus</i> EDS32332.1) sNPF and the predicted sNPF from the ant species <i>Attce (Atta cephalotes), Linhu (Linepithema humile),</i> and <i>Pogba (Pogonomyrmex barbatus)</i>. The honey bee, <i>Apime</i> NPY (NP_001161192.1) was also included in the analysis.</p