Detection of the fire ant sNPF (Partial sequence: ALAAGH) by MALDI-TOF MS.

Abstract

<p>The Y-axis represents % intensity (as % of the highest charge detected) and the X-axis represents mass. The agarose electrophoresis analysis shows the amplified expected size band (377 bp) of the fire ant sNPF from the fire ant larvae cDNA. The peptide sequence analysis performed with the PeptideCutter tool at ExPASy Bioinformatics Resource Portal revealed several potential protease cleavage sites, such as those for proteinase K (thin arrows), thermolysin (thick arrows), and Arg-C proteinase, Clostripain, and trypsin (black arrow head). The detected fragment is predicted after thermolysis digestion.</p