MiR-615-3p inhibits CHOP expression.

Abstract

<p>(A) Representative western blot for CHOP in IRE-WT cells treated with 400 µM palmitate (PA) or 1 µg/mL tunicamycin (tuni) for 16 hours. The cells were transfected with either a negative control, or a precursor of miR-615-3p (pre-miR-615-3p). Molecular weights are indicated in kDa. The immune complexes were detected using an infrared fluorescent imaging system. Alpha-tubulin was used as loading control. (B) Quantification of CHOP protein levels normalized to tubulin, under the same conditions as A, expressed relative to the negative control mimic treated cells for each condition, respectively. ** P<0.01. (C) Representative western blot for CHOP in Hepa 1–6 cells treated with 400 µM palmitate (PA) or 1 µg/mL tunicamycin for 24 hours. The cells were transfected with either a negative control, or a precursor of miR-615-3p (pre-miR-615-3p). Molecular weights are indicated in kDa. The immune complexes were detected using enhanced chemiluminescence. Alpha-tubulin was used as loading control. The middle panel (**) depicts a longer film exposure of the top panel (*) (D) Quantification of CHOP protein levels normalized to tubulin, under the same conditions as C, expressed relative to the negative control mimic treated cells for each condition, respectively. * P<0.05, ** P<0.01 (E) Representative western blot for CHOP in IRE-WT and Hepa1-6 cells in cells transfected with either an antagomir to miR-615-p or a negative control antagomir. Alpha-tubulin was used as loading control. (F) Quantification of CHOP protein levels normalized to tubulin, under the same conditions as E, expressed relative to the negative control antagomir treated cells for each condition, respectively. P = ns.</p

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