HaRxL62 reduces responsiveness to SA.

Abstract

<p>(A) Expression level of <i>PR1</i> 8 hours after treatment with SA (100 µM) in ten-day-old Col-0 plants (WT), <i>npr1</i> mutants and transgenic lines expressing the indicated <i>Hpa</i> predicted effectors. The expression level was determined by qRT-PCR using specific primers for <i>PR1</i> and indicated as relative fold induction compared to the expression level in WT after SA treatment. Expression of <i>EF-1α</i> was used to normalize the expression value in each sample. Data are means from three biological replicates showing quantiles. Data analysis was carried using one-way ANOVA followed by Tukey's HSD (honestly significant difference). Genotypes showing significant differences (<i>p</i><0.01) are marked with different alphabets (B) <i>Hpa</i> growth on three-week-old Col-0 plants (WT), <i>npr1</i> mutants and two independent transgenic lines expressing <i>HaRxL62</i> (HaRxL62-1 and HaRxL62-2) and <i>HaRxLL464</i> (HaRxLL464-1 and HaRxLL464-2) pretreated with SA (10 µM) or water (mock). The plants 24 hours after spray treatment with SA or water were inoculated with <i>Hpa</i> Waco9. Conidiospores were harvested and counted at 6 dpi. Different letters indicate significantly different values at <i>p</i><0.05 (one-way ANOVA, Tukey's HSD).</p