PI3K–Akt signaling in Oo<i>Rptor</i>^−/− and Oo<i>Rptor</i>^+/+ oocytes.

Abstract

<p>Oocytes were isolated from the ovaries of Oo<i>Rptor</i>^−/− and Oo<i>Rptor</i>^+/+ mice at PD12–14, and western blots were performed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110491#s4" target="_blank">Materials and Methods</a>. Levels of phosphorylation of Akt at S473 and T308 are elevated in Oo<i>Rptor</i>^−/− oocytes compared to Oo<i>Rptor</i>^+/+ oocytes, and this indicates that PI3K–Akt signaling in Oo<i>Rptor</i>^−/− oocytes is enhanced. Levels of total Akt and β-actin were used as internal controls.</p