<p>(<b>A and B</b>) <i>lin-35</i>/Rb mutant larvae were stained with DAPI (red) and anti-SPD-2 (green) to monitor centriole dynamics at the nuclear division. The panel (A) was obtained by staining animals approximately one hour after the nuclear division (t = 1 h), while (B) was acquired two hours after the division (t = 2 h). Asterisks indicate the intestinal nuclei and the arrowheads indicate SPD-2 foci. (<b>C and D</b>) <i>lin-35</i>/Rb mutant larvae were stained with DAPI (red) and anti-SAS-4 (green) to monitor centriole numbers after the nuclear division. The insets in (A–D) represent magnified views of regions highlighted by the white rectangles. Scale bar, 5 µm. (<b>E</b>) Quantification of SPD-2 or SAS-4 foci in intestinal nuclei in both wild type and <i>lin-35</i>/Rb mutants two hours after the first intestinal nuclear division. n = 75. (<b>F</b>) RT-PCR analysis of cell-specific transcripts from N2 and <i>lin-35</i> (<i>n745</i>). <i>elt-2</i> is intestinal specific, while <i>htp-3</i> is expressed exclusively in the germ line. (<b>G</b>) The expression of <i>spd-2</i>, -<i>5</i>, <i>zyg-1</i>, -<i>9</i>, <i>sas-4</i>, <i>-5</i>, <i>-6</i>, <i>tbg-1</i> and <i>dlg-1</i> (control) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110958#pone.0110958-McMahon1" target="_blank">[77]</a> was quantified using RT-PCR from total or intestine-enriched mRNA from wild type (N2) or from <i>lin-35 (n745)</i> larvae before or after the first nuclear division, and 6–8 hours after the last nuclear division in <i>lin-35</i> (<i>n745</i>) mutants. int., intestinal. bp, base pair.</p
