<p>(<b>A–C</b>) <i>spd-2</i> (<i>oj29</i>) animals carrying the SPD-2<sup>S357E</sup> variant were stained with DAPI (red) and anti-SPD-2 (green) in the L1, L1/L2 and L2. The asterisks indicate the intestinal nuclei. Scale bar, 5 µm. (<b>D</b>) SPD-2 staining was monitored in intestinal cells and the percentage of intestinal nuclei that demonstrate nuclear-localized SPD-2 in strains carrying either wild type SPD-2 or SPD-2<sup>S357E</sup> variant were determined. (<b>E</b>) The frequency of SPD-2 persistence is quantified by counting the number of intestinal cells that continue to show any SPD-2 signal at later larval stages. Error bar, standard deviation; n≥50; P<0.05 (t-test). (<b>F</b>) Late L2 <i>spd-2</i> (<i>oj29</i>) animals expressing the SPD-2<sup>WT</sup> or (<b>G</b>) the SPD-2<sup>S357E</sup> variant were stained with DAPI (red) and anti-SAS-4 (green). The number of SAS-4 foci were quantified and indicated in (<b>H</b>). The asterisks indicate the intestinal nuclei while arrowheads show SAS-4 foci. Error bar, standard deviation; n = 50; P<0.05 (t-test). Scale bar, 5 µm.</p
