Promoter elements of the <i>bla</i>_KPC-15 and <i>bla</i>_TEM genes in 8.997 kb-length nucleotide sequence.

Abstract

<p>The sequence provided the upstream and downstream regions of <i>bla</i>_KPC-15 structural genes. The <i>tnpA</i> gene, which was upstream of the <i>bla</i>_KPC-15 (3,020 bp) gene, was homologous to a putative IS<i>Kpn8</i> transposon, and the downstream region of the <i>bla</i>_KPC-15 gene (1,320 bp) was homologous to a putative IS<i>Kpn6</i>-like transposon. The nucleotides upstream of the <i>bla</i>_KPC-15 and <i>bla</i>_TEM-12 gene translational start codons were shown in the box. The putative −10 promoter elements of the <i>bla</i>_KPC-15 gene were shown as <i>gattaa</i>, labeled as −10 below, and there were no obvious −35 promoter elements to be discovered in the promoter region. The putative −10 and −35 promoter elements of the <i>bla</i>_TEM-12 gene were shown as <i>tataac</i> and <i>ttattg</i>, labeled as −10 and −35 below the promoter region. The start sites of transcription were indicated as G by +1 residue. RBS was the abbreviation of the ribosome binding site.</p