<p>(<b>A</b>) Diagram of SPD-2 and its potential phosphorylated sites. Numbers represent amino acid position. S, Serine. T, Threonine. Orange numbers: predicted consensus PLK phosphorylation site. Blue numbers: predicted consensus CDK phosphorylation site. Blue or Orange S indicates experimentally-confirmed phosphorylated Serine <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110958#pone.0110958-Bodenmiller1" target="_blank">[58]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110958#pone.0110958-Zielinska1" target="_blank">[78]</a>. (<b>B–D</b>) and (<b>E–G</b>) Early L2 <i>spd-2</i> (<i>oj29</i>) animals carrying transgenic WT or the S545-variant SPD-2 following the intestinal nuclear division. DAPI (red) and SPD-2 (green) in (B–D) or SAS-4 (green) in (E–G). Asterisks indicate the intestinal nuclei and arrowheads show SPD-2 or SAS-4 foci. The insets show high magnification of the regions within the white rectangles. Scale bar, 5 µm. (<b>H</b>) The frequency of centriole duplication failure is represented by quantifying undivided intestinal nuclei associated with single SPD-2 or SAS-4 foci. (<b>I</b>) The frequency of supernumerary centriole duplication is indicated by the number of divided intestinal nuclei with more than one SPD-2 or SAS-4 focus after the nuclear division. Error bar, standard deviation; n≥75; P<0.05 (t-test). (<b>K</b>) Mass spectrometric analysis of SPD-2. +80 indicates the phosphorylated amino acid and the arrow highlights S545 in red.</p
