Pterostilbene and 3′-hydroxypterostilbene reduced the growth of COLO 205 xenografts in nude mice.

Abstract

<p>Experimental treatment protocol as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111814#s2" target="_blank">Materials and Methods</a>. Mice bearing COLO 205 xenografts were <i>i.p.</i> injection with pterostilbene or 3′-hydroxypterostilbene for 15 days where control group was received corn oil. (A) Photograph of xenograft tumors developed in each group is shown at the end of day15. (B) Average tumor volumes were recorded during the treatment and (C) average tumor weights were measured at the end of experiment. Six samples were analyzed in each group, and values represent the mean ±SD. ^*<i>P</i><0.05 and ^**<i>P</i><0.01, compared with control group. ^#<i>P</i><0.05, compared with pterostilbene-treated group. (D) Total proteins of xenograft tumors in each group were extracted for western blot analysis. COX-2, MMP-9, VEGF, PCNA, cyclin D1 protein expression and cleaved caspase-3 were detected by using specific antibodies. Similar results were obtained in three independent experiments.</p