p23 Hsp90 co-chaperone is a modulator of the MSR.

Abstract

<p>(A) Quantification of HSF1-P after 1 h HS in F508del-expressing cells treated with control or p23 siRNA. Data is shown as percentage of control (mean ± SEM, n = 4). (B) Immunoblot of the indicated proteins following p23 silencing in F508del-CFTR expressing cells. Histograms represent quantification of the indicated proteins upon sip23, relative to levels seen with control siRNA, which is set to 100%. (C) qRT-PCR analysis of I-Hsp70 in F508del-expressing cells following sip23. The data represent a ratio of I-Hsp70 to the housekeeping gene (GUS) and are shown as percentage of control siRNA. All results are shown (B,C) as a mean ± SEM, n≥3, and * indicates <i>p</i><0.05 relative to control. (D) Quantification of HSF-1-P and I-Hsp70 protein levels in WT- and F508del- expressing cells after control or sip23 treatment. The data is shown as a percentage of F508del-expressing cells, and # represents <i>p</i><0.05 relative to F508del (mean ± SEM, n≥3). (E) Quantitative analysis of FLuc activity in WT- and F508del-CFTR expressing cells treated with control or sip23. The data represents normalized specific activity of FLuc (luminescence/relative FLuc expression) for each condition (mean ± SEM, n≥3). All results were replicated at least once. The symbols * and # represent <i>p</i><0.05 relative to WT and F508del respectively. The underlying data used to make (A–E) in this figure can be found in the supplementary file <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001998#pbio.1001998.s008" target="_blank">Data S1</a>.</p