Measured miRNAs in matched serum and plasma.
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Abstract
<p>Expression levels of miR-21, miR-29a, miR-125b and miR-16 (<b>A</b>) and the degree of hemolysis (<b>B</b>) are illustrated. Absorbance of each serum sample at 560 nm, 576 nm and 592 nm was measured by spectrophotometry and the degree of hemolysis was calculated by the following formula: estimated hemoglobin level = 2*OD<sup>576 nm</sup>−OD<sup>560 nm</sup>−OD<sup>592 nm</sup>. Differences between serum and plasma were analyzed by the Wilcoxon signed-rank test.</p