The MAK-2 pathway elements STE-20 and RAS-2 are important for cell-cell communication.

Abstract

<p>(<b>A</b>) STE-20-GFP localizes in a stable manner to the apices of two communicating germlings and marks the site of contact. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004762#pgen.1004762.s015" target="_blank">Video S6</a> for time course. (<b>B</b>) GFP-RAS-2 associates with the entire plasma membrane of germinating spores and localizes at the contact point of two communicating cells. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004762#pgen.1004762.s016" target="_blank">Video S7</a> for time course. (<b>C</b>) Time course experiments to determine rates of germination and of chemotropic interactions of the indicated mutants. Note that due to delayed germination Δ<i>cr-1</i> and Δ<i>cap-1</i> strains are assayed at later time points than the other mutants. (<b>D</b>) Quantification of basal and stress-induced MAK-2 phosphorylation levels (detected with p42/44 antibodies) in cell extracts of exponentially growing liquid cultures of the indicated strains (n = 3). A representative Western blot is depicted below; tubulin was used as loading control.</p

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