Interaction network of MAK-2 pathway components.

Abstract

<p>(<b>A</b>) Proteins associated with the MAK-2 cascade were identified in affinity purification experiments coupled to mass spectrometry (AP-MS), and identified proteins were filtered against control purifications using GFP as bait (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004762#pgen.1004762.s007" target="_blank">Table S1</a>). Only proteins identified in two biological replicates and absent from the control data set are shown. Protein coverage by peptides and the number of identified total and unique peptides identified are given for the better of the two purifications. Bold numbers indicate the GFP-fusion protein used as bait. (<b>B</b>) Physical interactions between MAK-2 pathway components were mapped in yeast two-hybrid (Y2H) tests. The indicated constructs were co-expressed in strain AH109 and yeast growth was analyzed on the indicated selective media. The reciprocal Y2H assays are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004762#pgen.1004762.s002" target="_blank">Figure S2</a>. (<b>C</b>) Summary schema of Y2H-based interactions of the indicated proteins and their domains. Color-coded lines below the protein schemas and dashed connectors indicate the used constructs and detected interactions, respectively.</p

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