STE-50 functions as regulatory subunit of NRC-1.

Abstract

<p>(<b>A</b>) GFP-STE-50 localizes in a dynamic manner to opposing tips of two communicating germlings and the site of cell-cell contact. The oscillation period is approximately three to five minutes. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004762#pgen.1004762.s010" target="_blank">Video S1</a> for time course. (<b>B</b>) GFP-STE-50 localizes in a diffuse cytosolic manner in non-communicating hyphae and is excluded from nuclei (left image). Moreover, GFP-STE-50 accumulates at septa (arrowheads) and the contact point (arrow) of communicating hyphal tips (right image). (<b>C</b>) Δ<i>ste-50</i> fully phenocopies Δ<i>mak-2</i> defects (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004762#pgen.1004762.s003" target="_blank">Figure S3</a> for comparative characterization of mutant defects), and the mutant characteristics are complemented by expression of a constitutive active NRC-1(P488S) allele in Δ<i>ste-50</i>. The slant images represent macroscopic appearance and conidiation pattern, while the Table summarizes rates of mycelial extension, protoperithecia (PP) formation, germling communication and MAK-2 activities of the indicated strains.</p

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