<p>The reaction mixture in a total volume 1 ml contained (final concentration): malonate buffer (20 mM, pH 4.5), Mn<sup>2+</sup> (1.6 mM), H<sub>2</sub>O<sub>2</sub> (0.08 mM), purified CD2-MnP (0.25 U/ml), dye (50 mg/L) and Ca<sup>2+</sup>, Co<sup>2+</sup>, Mg<sup>2+</sup>, Zn<sup>2+</sup> (4 mM). <b>CK (MnP+H<sub>2</sub>O<sub>2</sub>)</b> was the control without addition of any metal compound except Mn<sup>2+</sup>. <b>H<sub>2</sub>O<sub>2</sub> (no MnP)</b> was the negative control without addition of purified CD2-MnP. (<b>A</b>): Decolorization of RBV5R; (<b>B</b>): Decolorization of DR5B; (<b>C</b>): Decolorization of RBBR; (<b>D</b>): Decolorization of IC; (<b>E</b>): Decolorization of MG. <b>RBV5R</b>: Remazol Brilliant Violet 5R, <b>DR5B</b>: Direct Red 5B, <b>RBBR</b>: Remazol Brilliant Blue R, <b>IC</b>: Indigo Carmine, <b>MG</b>: Methyl Green. The negative control (H<sub>2</sub>O<sub>2</sub> was added into the decolorization mixture in the absence of purified CD2-MnP) showed no significant decolorization of different dyes.</p
