Results of osmotic protection experiments with HlyA, HlyA<sub>Δ71–110</sub>, and HlyA<sub>Δ264–286</sub>.
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Abstract
<p>Sheep erythrocytes were incubated with the toxins at 37°C for 60 min in saline solution (control) or in saline solution supplemented with 30 mM of PEGs of different molecular masses (PEG 400, 600, 1000, 2000, 3000, and 4000, with diameters of 1.07, 1.32, 1.72, 2.47, 3.05, and 3.54 nm, respectively). The concentration of HlyA was 0.5 µg/ml and that of HlyA<sub>Δ71–110</sub> and HlyA<sub>Δ264–286</sub> 2.5 µg/ml. The degree of hemolysis is shown as a function of the molecular mass of the PEGs.</p
