Single-channel recordings with <i>E. coli</i> HlyA, HlyA<sub>Δ71–110</sub>, and HlyA<sub>Δ264–286</sub>.
- Publication date
- Publisher
Abstract
<p>Single-channel recordings of asolectin membranes were performed in the presence of 50 ng/ml HlyA (left side, upper trace), 50 ng/ml HlyA<sub>Δ71–110</sub> (left side, lower trace), and 50 ng/ml HlyA<sub>Δ264–286</sub> (right side). The aqueous phase contained 150 mM KCl (pH 6). The applied membrane potential was 20 mV; T = 20°C. The average single-channel conductance was 520 pS for HlyA, 150 pS for HlyA<sub>Δ71–110</sub>, and 320 pS for HlyA<sub>Δ264–286</sub>.</p
