Average single-channel conductance, <i>G</i>, of HlyA_Δ71–110 and HlyA_Δ264–286 in different salt solutions.^a.

Abstract

a<p>The membranes were formed from 1% (mass/volume) asolectin dissolved in n-decane. The aqueous solutions were unbuffered and had a pH of 6. The applied voltage was 20 mV, and the temperature was 20°C. The average single-channel conductance, <i>G</i> (i.e. current divided by voltage), was calculated from at least 80 single events. The standard deviation of the single-channel conductance was generally below ±15%. <i>c</i> is the concentration of the aqueous salt solutions. The single-channel conductance of wildtype HlyA of <i>E. coli</i> is given for comparison <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112248#pone.0112248-Benz3" target="_blank">[28]</a>. The values denoted with an asterix were measured during this study with purified HlyA. n.m. means not measured.</p><p>Average single-channel conductance, <i>G</i>, of HlyA_Δ71–110 and HlyA_Δ264–286 in different salt solutions.^a.</p