Single-channel recordings with HlyA<sub>Δ158–167</sub> and HlyA<sub>Δ180–203</sub>.
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Abstract
<p>Single-channel recordings of asolectin membranes were performed in the presence of 100 ng/ml HlyA<sub>Δ158–167</sub> (upper trace) and 150 ng/ml HlyA<sub>Δ180–203</sub> (lower trace). The aqueous phase contained 150 mM KCl (pH 6). The applied membrane potential was 20 mV; T = 20°C. The transient conductance steps in the upper trace (HlyA<sub>Δ158–167</sub>) had a conductance of about 600 to 700 pS. The mutant HlyA<sub>Δ180–203</sub> produced under the given conditions only current noise (fuzzy channels) and no defined conductance states.</p
