PRL1 is required for miRNA maturation <i>in vitro</i>.

Abstract

<p>(A) and (B) A schematic diagram of the <i>MIR162b</i> (A) and <i>pre-miR162b</i> (B) used <i>in vitro</i> processing assay. (C) and (D) The amount of miR162b produced from <i>MIR162b</i> and <i>pre-miR162b</i> were reduced in <i>prl1-2</i>. Proteins were isolated from inflorescences of <i>prl1-2</i> and Col and incubated with <i>MIR162b</i> or <i>pre-miR162b</i>. The reactions were stopped at various time points as indicated in the picture. (E) and (F) Quantification of miR162b production in <i>prl1-2</i> compared to that in Col. Quantification analysis was performed at 80 min. The radioactive signal of miR162 were normalized to input and compared with that of Col. The amount of miR162 produced in Col was set as 1. The value represents mean of three repeats (*** <i>P</i><0.001; t-test).</p