Zebrafish Abcc4 plays crucial roles in cellular detoxification of DDT and lindane.

Abstract

<p>(<b>A</b>) Death rates of 96-hpf embryos expressing GFP, Abcc4 or Abcc4-G1188D were monitored after treatment with 100 µg/L DDT or lindane. (B) Embryos injected with pT2/CMV2-Abcc4 or pT2/CMV2-GFP were exposed to 100 µg/L DDT or lindane from 24 to 96 hpf. Eighty embryos at 96-hpf stages were pooled for total RNA extraction and subjected to real-time PCR analysis. (<b>C and D</b>) Survival rates of LLC-PK1 cells expressing Abcc4 or Abcc4-G1188D, transfected empty vectors (CTRL) and treated with 25 µM MK571 were determined with MTT assays after exposure to DDT or lindane at indicated concentrations for 24 hours. Values are expressed as mean ± standard deviation (n = 3). Significant differences are indicated by ^*<i>p</i><0.05 and ^**<i>p</i><0.01.</p