Abstract

<p>K562 cells were subjected to one round of transduction with the lentiviruses indicated in the figure. After 5 days the cells were sorted by FACSAriaIII based on EGFP expression (<b>A</b>), and the sorted EGFP-positive (gates 1, 3, 5, 7) and -negative (gates 2, 4, 6, 8) subpopulations were analyzed by western blotting for expression of 3xFLAG-ZNF521 and of EGFP (<b>B</b>). The purity of the sorted populations was subsequently evaluated by flow cytometry and is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114795#pone.0114795.s003" target="_blank">S3 Figure</a>.</p

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