Enhanced Resolution of Low Molecular Weight Poly(Ethylene
Glycol) in Nanopore Analysis
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Abstract
A design with conjugation of DNA
hairpin structure to the poly(ethylene
glycol) molecule was presented to enhance the temporal resolution
of low molecular weight poly(ethylene glycol) in nanopore studies.
By the virtue of this design, detection of an individual PEG with
molecular weight as low as 140 Da was achieved at the single-molecule
level in solution, which provides a novel strategy for characterization
of an individual small molecule within a nanopore. Furthermore, we
found that the current duration time of poly(ethylene glycol) was
scaled with the relative molecular weight, which has a potential application
in single-molecule detection