Enhanced Resolution of Low Molecular Weight Poly(Ethylene Glycol) in Nanopore Analysis

Abstract

A design with conjugation of DNA hairpin structure to the poly­(ethylene glycol) molecule was presented to enhance the temporal resolution of low molecular weight poly­(ethylene glycol) in nanopore studies. By the virtue of this design, detection of an individual PEG with molecular weight as low as 140 Da was achieved at the single-molecule level in solution, which provides a novel strategy for characterization of an individual small molecule within a nanopore. Furthermore, we found that the current duration time of poly­(ethylene glycol) was scaled with the relative molecular weight, which has a potential application in single-molecule detection

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