T7 endonuclease I (T7EI) analysis of potential off-target loci.
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Abstract
<p>A. T7EI analysis of three potential off-target loci (AKAP9, ULK1 and MED16) to CR2 as well as on-target locus CCR5. “–”: genomic DNA isolated from mock-transduced TZM.bl; “+”: genomic DNA isolated from sorted CCR5-/CR2 cells. D21 and D84: genomic DNA samples isolated from sorted CCR5-/CR2 cells at 21 and 84 days post transduction. B. T7EI analysis of nine potential off-target loci (SH2D5, ASB9P1, PRRT1, LINC00265, ENDOV, NR2F1, ASB9, CLPP and SOBP) to CR3 as well as on-target locus CCR5 control. “–”: genomic DNA isolated from parental TZM.bl; “+”: genomic DNA isolated from sorted CCR5-/CR3 cells. D21 and D84: genomic DNA samples isolated from sorted CCR5-/CR3 cells at 21 and 84 days post transduction.</p