Induction of reactive oxygen species in kidney and heart.

Abstract

<p>ROS formation quantified after staining kidney <b>(a)</b> and heart <b>(b)</b> cryosections with the ROS-sensitive fluorescent dye dihydroethidium. Quantification was done with the free software Cell Profiler <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115715#pone.0115715-Lamprecht1" target="_blank">[15]</a>. Data are shown as mean ± SEM after normalization to control values. Western blot-analysis of the amount of one subunit of the ROS-generating enzyme NADPH-oxidase 4 (NOX4) in protein extracts of kidney <b>(c)</b> and heart <b>(d)</b>, related to the house-keeping protein glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Shown are representative blots and the quantification of band densities of proteins of all animals. Relative quantification of the transcripts of the NADPH-oxidase subunits 1 (NOX1), 2 (NOX2) and 4 (NOX4) in kidney tissue <b>(e)</b>. Western blot-analysis of the amount of xanthine oxidase in protein extracts of the heart <b>(f)</b> related to the house-keeping protein glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Shown is a representative blot and the quantification of band densities of proteins of all animals. * p≤0.05, ** p<0.01, *** p<0.001 vs. Control, °° p<0.01 vs. AngII-treatment.</p

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