[Au(dien)(N-heterocycle)]<sup>3+</sup>: Reactivity
with Biomolecules and Zinc Finger Peptides
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Abstract
The
reaction of [Au(dien)(N-heterocycle)]<sup>3+</sup> (AuN<sub>4</sub>) coordination compounds with simple amino acids and zinc
finger proteins is reported. Compared to [AuCl(dien)]<sup>2+</sup>, NMR studies show that the presence of a more substitution-inert
N-donor as the putative leaving group slows the reaction with the
sulfur-containing amino acids <i>N</i>-acetylmethionine
(NAcMet) and <i>N</i>-acetylcysteine (NAcCys). Lack of ligand
dissociation upon reaction with NAcCys indicates, to our knowledge,
the first long-lived N-heterocycle–Au–S species in solution.
Reactions with zinc finger proteins show a higher reactivity with
the Cys<sub>3</sub>His zinc finger than with Cys<sub>2</sub>His<sub>2</sub>, likely due to the presence of fewer aurophilic cysteines
in the latter. Of the Au(III) compounds studied, [Au(dien)(DMAP)]<sup>3+</sup> (DMAP = 4-dimethylaminopyridine) appears to be the least
reactive, with ESI-MS studies showing the presence of intact zinc
fingers at initial reaction times. These results, in combination with
previously reported characterization and pH dependency studies, will
further aid in optimizing the structure of these AuN<sub>4</sub> species
to obtain a substitution-reactive yet selective compound for targeting
zinc finger proteins