<p>A) The figure shows β-galactosidase activity measurements for lysates obtained from cultures of M182 or the Δ<i>hns</i> and Δ<i>crp</i> derivatives containing P<i>estA2</i> (460 bp fragment) or P<i>eltAB</i> (1126 bp fragment) from ETEC 24377A fused to <i>lacZ</i> in plasmid pRW50. B) The panel shows log<sub>2</sub> fold changes in the transcription of <i>crp</i>, <i>hns</i>, <i>eltA</i> and <i>estA</i> in ETEC E24377A cells over a two hour incubation with a Caco-2 intestinal epithelial cell culture (29). The log<sub>2</sub> values represent the fold change in transcription between ETEC cells attached and unattached to Caco-2 cells at each time point. C) The panel shows a scatter plot of absolute <i>crp</i> and <i>estA2</i> mRNA levels in ETEC E24377A attached to Caco-2 intestinal epithelial cells. Each data point represents a different biological replicate. For each data point the absolute level of <i>hns</i> mRNA is shown in parenthesis. D) The panel shows the survival rate of BALB/C mice (n = 30) after intranasal inoculation with wild type ETEC H10407 or the Δ<i>crp</i> derivative.</p
