SDS-PAGE analysis of the purification of WcE392-rDSR.

Abstract

<p>Gel A shows the purification protocol of the enzyme from <i>L. lactis</i> culture supernatant (1). A 2-fold concentrate obtained by ultrafiltration and the ultrafiltration permeate are shown on lanes 2 and 3, respectively. Lane 4 shows the sample eluted from Ni-NTA column. Gel B shows the approximately 60-fold concentrated enzyme sample obtained by ultrafiltration and dilution.</p