Denaturation of erythroid and nonerythroid spectrin monitored by CD spectroscopy.

Abstract

<p>CD spectra of (A) erythroid spectrin and (B) nonerythroid spectrin showing the native (a) and the unfolded proteins in 8M urea (b) and 6M GuHCl (c). Urea and GuHCl induced changes in secondary structure of (C) erythroid spectrin and (D) nonerythroid spectrin by changes in ellipticity at 222 nm with increasing concentrations of the denaturants. Effects of urea and GuHCl on erythroid, non-erythroid spectrin (0.2 μM each) are shown by changes in ANS fluorescence at 470nm in (E) erythroid and (F) nonerythroid spectrin respectively.</p