STAT3 signaling pathway was involved in the inhibitory effect of ghrelin on Th17 cells.
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Abstract
<p>(A) Total T cells were isolated from spleens of GHSR1a<sup>WT</sup> and GHSR1a<sup>-/-</sup> mice. The phosphorylation of STAT3 was analyzed with Western Blot. (B) Total T cells were isolated from mouse spleens and induced differentiation to Th17 cells, then treated with ghrelin (10<sup>–8</sup> M). The phosphorylation of STAT3 was analyzed with Western Blot. (C) Differentiated Th17 cells were pre-treated with or without Colivelin (100 pM), then treated with or without ghrelin (10<sup>-8</sup>M). The phosphorylation of STAT3 was analyzed with Western Blot. Relative protein signal intensity was quantified. (D&E) The mRNA level of RORγt (D) and IL-17A (E) was analyzed with RT-PCR. (F) The concentration of IL-17A in the supernatant was examined with ELISA. (G&H) The percentage of IL-17A<sup>+</sup> cells in splenic total T cells (G) and CD4<sup>+</sup> T cells (H) was analyzed with flow cytometry. Shown is the representative of three independent experiments. *<i>P</i><0.05 versus control; <sup>#</sup><i>P</i><0.05 versus ghrelin-treated alone.</p