Leucine rescued the inhibitory effect of ghrelin on Th17 cells.

Abstract

<p>Total T cells were isolated from mouse spleens and induced differentiation to Th17 cells. (A) Differentiated Th17 cells were treated with ghrelin (10<sup>–8</sup> M) and leucine (1 mM). The phosphorylation of S6 was analyzed with Western Blot. Relative protein signal intensity was quantified. (B&C) Differentiated Th17 cells were treated with ghrelin (10<sup>–8</sup> M) and leucine with final concentration as indicated. The mRNA levels of RORγt (B) and IL-17A (C) were analyzed with RT-PCR. (D) Differentiated Th17 cells were treated with ghrelin (10<sup>–8</sup> M) and leucine (1 mM). The concentration of IL-17A in the supernatant was examined with ELISA. (E&F) The percentage of IL-17A<sup>+</sup> cells in splenic total T cells (E) and CD4<sup>+</sup> T cells (F) was analyzed with flow cytometry. Shown is the representative of three independent experiments. *<i>P</i><0.05 versus control; <sup>#</sup><i>P</i><0.05 versus ghrelin-treated alone.</p

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