Neutrophil migration is dose dependent in both the gradient and non-gradient assays.

Abstract

<p>a) migration in response to CXCL8, *significantly greater as compared to control cells, p<0.05, n = 3 except for non gradient control and 100ng/ml where n = 5. b) The direction of movement for each neutrophil in the non gradient and gradient assay in response to 100ng/ml of CXCL8 is illustrated in the vector diagrams which show chemokinetic migration in the non-gradient assay and a chemotactic pattern of migration in the gradient assay. c) Neutrophil migration to GM-CSF is dose dependent in both the non gradient and gradients assays. *significantly greater as compared to control cells, p<0.05, n = 3 except for gradient control and 50ng/ml where n = 7 and 6 respectively. d). The direction of movement for each neutrophil in the non gradient and gradient assay in response to 50ng/ml of GM-CSF is illustrated in the vector diagrams, These show that the pattern of migration to GM-CSF was chemokinetic in both the gradient and non-gradient assays.</p