Normal brain development in <i>Lrp4</i>^{<i>ECD/ECD</i>} and <i>Lrp4</i>^<i>-/-</i> mice.

Abstract

<p><b>A-D</b>: Sagittal slices of the <i>Lrp4</i>^{<i>ECD/ECD</i>} (A,C) and wild type (<i>Lrp4</i>^<i>+/+</i> B,D) mouse cerebellum labeled with NeuN (green), Brn1 (red) and DAPI (blue). Brn1 and NeuN are commonly used markers to label neurons. ML = molecular layer, PL = Purkinje cell layer, and GCL = granule cell layer are clearly distinguishable and not different in the cerebellum of <i>Lrp4</i>^{<i>ECD/ECD</i>} and <i>Lrp4</i>^<i>+/+</i> adult mice (>2 months). <b>E-H</b>: Coronal sections of <i>Lrp4</i>^{<i>ECD/ECD</i>} (E,F) and <i>Lrp4</i>^<i>+/+</i> (G,H) brains showing hippocampus (E,G) and somatosensory cortex (F,H). Slices are labeled for NeuN and DAPI to visualize normal cortical lamination (layers I-VI). I-N: Coronal sections of E18.5 <i>Lrp4</i>^<i>-/-</i> brains compared to their wild type litter mates. Brn1 (I,J) and GFAP (K,L) immunoreactivity in the cortex and hippocampus and Tbr1 plus NeuN double labeling in the cortex are illustrated. Scale bars = 200 μm (A-H), 400 μm (I-L), 100 μm (M,N).</p