IPP and VPP promote expression of the adipocyte differentiation markers PPARγ and adiponectin in 3T3-F442A cells.

Abstract

<p>3T3-F442A cells were incubated in presence of insulin (10 μg/mL), IPP (50 μM) or VPP (50 μM) for 72 hr. (A) The cells were lysed and western blotting of the lysates was performed with antibodies against PPARγ and α-tubulin (loading control). A set of representative images (including cropped images obtained from the same membrane) was shown. (B) The cell-free culture supernatants were collected and analyzed by ELISA to determine levels of adiponectin. Data were presented as mean±SEM of 5 independent experiments. * and *** indicate p<0.05 and p<0.001 compared to the untreated control (Untr) respectively.</p