IPP and VPP induce lipid accumulation in 3T3-F442A cells.

Abstract

<p>3T3-F442A cells were incubated in presence of insulin (10 μg/mL), IPP (50 μM) or VPP (50 μM) for 72 hr. (A) For one study, the cells were fixed, stained with the neutral lipid-specific dye LipidTox and visualized under fluorescence microscopy. A set of representative images are shown. (B) For another set of experiments, the cells were lysed and their lipid contents were estimated by a biochemical assay. Data were presented as mean±SEM of 3–4 independent experiments. * and ** indicate p<0.05 and p<0.01 respectively compared to the untreated control (Untr).</p