Cytosolic and mitochondrial ferritin in FA cardiomyopathy.

Abstract

<p>(a) and (b), positive contrast immunohistochemistry of adjacent sections (LVW, FA patient FA10, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116396#pone.0116396.t001" target="_blank">Table 1</a>); (c-h), laser scanning confocal immunofluorescence (LVW, FA patient FA7, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116396#pone.0116396.t001" target="_blank">Table 1</a>). (a), cytosolic ferritin; (b) mitochondrial ferritin; (c) and (f), cytosolic ferritin visualized by Cy3 (red); (d) and (g), mitochondrial ferritin visualized by Alexa 488 (green); (e) and (h) are merged images of (c) and (d), and (f) and (g), respectively. Cytosolic (a) and mitochondrial ferritin (b) show extensive co-localization of their immunohistochemical reaction products. In the series (c-e), three heart fibers display co-localization of cytosolic and mitochondrial ferritin. In the series (f-h), a single fiber is strongly fluorescent for cytosolic ferritin but does not express mitochondrial ferritin. Bars: (a-b), 100 μm; (c-h), 20 μm.</p