Representative Day 0 MRI, fluorescence microscopy, and histology acquired as 10x magnification from both implant models.

Abstract

<p>(A, E) Representative MRI from mice receiving either 2x10⁶ mMSC or 1.5x10⁶ hMSC respectively. The day 0 <i>in vivo</i>¹⁹F-MRI quantification correlates very well with the number of implanted cells. The reference tube is marked by “R”. (B) The red fluorescent fluorine agent is clearly visible in the tissue of the immune competent model, (F) as well as in the immune-compromised model. (C) Furthermore, the GFP+ mMSC are observable within the tissue section. (D) Overlaying the two fluorescent images, reveals the ¹⁹F agent colocalized with the GFP+ mMSC, as expected. (G, H) H&E stained tissue sections corresponding to the fluorescence microscopy clearly show the implant site of the mMSC and hMSC respectively. Scale bars in all images represent 250μm.</p