<p>(A) The expression pattern of <i>C316-GAL4</i> (green). The brain was immunostained with DLG antibody (red). Arrowheads indicate the somata of DPM neurons. The scale bar represents 50 μm. Genotype was as follow: <i>UAS-mCD8</i>::<i>GFP/+; C316-GAL4/UAS-mCD8</i>::<i>GFP</i>. (B) The expression pattern of <i>VT6412-GAL4</i> (green). The brain was immunostained with DLG antibody (red). Arrowheads indicate the somata of DPM neurons. The scale bar represents 50 μm. Genotype was as follow: <i>UAS-mCD8</i>::<i>GFP/+; VT64246-GAL4/UAS-mCD8</i>::<i>GFP</i>. (C) The expression pattern of <i>VT64246-GAL4</i> (green). The brain was immunostained with DLG antibody (red). Arrowheads indicate the somata of DPM neurons. The scale bar represents 50 μm. Genotype was as follow: <i>UAS-mCD8</i>::<i>GFP/+; VT64246-GAL4/UAS-mCD8</i>::<i>GFP</i>. (D) Overexpression of the <i>amn</i> transgene (<i>amn</i><sup><i>+</i></sup>) in DPM neurons reversed the deficiency of egg-laying decisions in <i>amn</i><sup><i>1</i></sup> background. Each value represents mean ± SEM (n = 22–24, ***P < 0.001, n.s., not statistically significant). Genotypes were as follows: (1) +/+, (2) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup>, (3) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup><i>; +/+; +/UAS-amn</i><sup><i>+</i></sup>, (4) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup><i>; +/+; C316-GAL4/+</i>, (5) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup><i>; +/+; VT6412-GAL4/+</i>, (6) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup><i>; +/+; VT64246-GAL4/+</i>, (7) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup><i>; +/+; C316-GAL4/UAS-amn</i><sup><i>+</i></sup>, (8) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup><i>; +/+; VT6412-GAL4/UAS-amn</i><sup><i>+</i></sup>, and (9) <i>amn</i><sup><i>1</i></sup><i>/amn</i><sup><i>1</i></sup><i>; +/+; VT64246-GAL4/UAS-amn</i><sup><i>+</i></sup>. (E) Effects of acute silencing of neuronal activity in DPM neurons on egg-laying decisions. The schematics of the temperature shift protocols are shown above each graph. Each value represents mean ± SEM (n = 17–26, ***P < 0.001. n.s., not statistically significant). Genotypes were as follows: (1) <i>+/+; VT6412-GAL4/+</i>, (2) <i>tubP-GAL80</i><sup><i>ts</i></sup><i>/+; UAS-Kir2</i>.<i>1/+</i>, and (3) <i>tubP-GAL80</i><sup><i>ts</i></sup><i>/+; VT6412-GAL4/UAS-Kir2</i>.<i>1</i>.</p
