PAEP gene knockdown in melanoma cells.

Abstract

<p>Stable PAEP 624.38-Mel shRNA transfectant and its non-targeting negative control transfectant, shControl, were established with a corresponding shRNA lentivirus. PAEP gene expressions were assayed by semi-quantitative RT-PCR (A) and Western blotting (B). PAEP protein secreted by melanoma cells was quantitated by ELISA (C).</p