<i>sox6</i> and <i>rod1</i> gene expression (miR-499 targets) in red and white skeletal muscle fibers of Nile tilapia.

Abstract

<p>RT-qPCR shows gene expression levels of <i>sox6</i>, <i>rod1</i>, and <i>myh7b</i> (miR-499 host gene) between muscle cell types (A). Relative quantification values are shown on Log 2 basis. *Statistical significance in comparison to white muscle (P<0.05). RM = red muscle; WM = white muscle; RQ = relative amount of transcripts. Fluorescent <i>in situ</i> hybridization demonstrates the spatial location of <i>sox6</i> mRNA at <i>200x</i> magnification (B and C) and <i>400x</i> magnification (D and E) and <i>rod1</i> mRNA at <i>200x</i> magnification (F and G) and <i>400x</i> magnification (H and I) in red and white muscle fibers.</p