Lal-/- cells (HD1B) stimulated cancer cells growth.

Abstract

<p>(A) Wild type (HD1A) and <i>lal</i>-/- (HD1B) cells were co-cultured with CFSE labeled LLC or B16 melanoma cells (5:1) for 3 days. The results are presented as percentage of CFSE positive cells increased from none co-culture base line. The results are mean ± SD from three independent experiments (n = 3), **, p< 0.01. (B) mTOR siRNA knockdown in <i>lal</i>-/- (HD1B) cells inhibited the stimulatory activity of cancer cell growth (LLC and B16 melanoma cells). The same cell ratio was used. The results are mean ± SD from three independent experiments (n = 3), **, p< 0.01; (C) B16 melanoma cells (2 X 10⁵) that were mixed with the same amount of wild type (HD1A) or <i>lal</i>-/- (HD1B) cells were left-side and right-side pair-injected subcutaneously into C57BL/6 or FVB/N mice. After 14 days, tumor volumes were measured using the formula: (length X width²) /2. The results are mean ± SD from 11–12 independent experiments (n = 11–12), p values are listed in comparison of tumor sizes between co-injection of wild type (HD1A) and <i>lal</i>-/- (HD1B) cells.</p