<p>(A) Western blot analyses of the phosphorylation level of mTOR downstream effector pS6 in wild type (HD1A) and <i>lal</i>-/- (HD1B) cells. Both cells were treated with solvent (S) or with mTOR inhibitor rapamycin (R) or PP242 (P). (B) Flow cytometry analyses of the ROS levels of rapamycin or PP242 treated or untreated wild type (HD1A) and <i>lal</i>-/- (HD1B) cells. Results are mean ± SD from three independent experiments (n = 3), p< 0.001. (C) Mitochondria membrane potential was analyzed in rapamycin or PP242 treated or untreated wild type (HD1A) and <i>lal</i>-/- (HD1B) cells by JC1 staining. Treatment of mTOR inhibitors restored the mitochondria membrane potential in <i>lal</i>-/- (HD1B) cells. (D) Antioxidant reagent NAC or Tempol treated or untreated wild type (HD1A) and <i>lal</i>-/- (HD1B) cells were stained with JC1 to measure the mitochondria membrane potential. Treatment of antioxidants restored the mitochondria membrane potential in <i>lal</i>-/- (HD1B) cells.</p
