GLI1 knockdown inhibits the cell proliferation, migration and invasion abilities in NB cells under hypoxic conditions.

Abstract

<p>(A) Three siRNAs targeting GLI1 and NC siRNA were transfected into SH-SY5Y cells. Western blotting indicated that siRNA2 provided the highest inhibition efficiency. (B, C) GLI1 knockdown significantly inhibited proliferation in IMR32 cells, but only led to a slight reduction in proliferation in SH-SY5Y cells (n = 9). (D, E) Decreased cell migration was observed in both cell lines transfected with GLI1 siRNA (n = 9). Magnification, ×40. (F, G) Invasion ability was reduced in both cell lines transfected with GLI1 siRNA (n = 9). Magnification, ×200. Data are expressed as means ± SD.</p