<p>(A) Western blot analyses showed the mTOR protein expression level, phosphorylated mTOR, and phosphorylated S6 levels reduced in wild type (HD1A) and <i>lal</i>-/- (HD1B) cells by mTOR siRNA knocking down. (B) T cell suppressive activity of HD1B was reduced upon mTOR knocking down by siRNA transfection. Wild type (HD1A) or <i>lal</i>-/- (HD1B) cells were pretreated with control (C) siRNA or mTOR (mTOR) siRNA. Treated cells were incubated with CFSE labeled wild type CD4<sup>+</sup> T cells and stimulated with anti-CD3 and anti-CD28 antibodies. Results are mean ± SD from three independent experiments (n = 3), p< 0.05; (C) Secretion of T cell releasing IL-4 in the above co-culture experiment was measured to assess the CD4<sup>+</sup> T cell function. Results are mean ± SD from three independent experiments (n = 3), p< 0.05.</p
