<p>(A) C-terminal sequencing of the larger cleaved fragment. The peptide mapping work was carried out using the NanoLC-MS/MS peptide sequencing technology. C-terminal peptide was identified: TLSRIEL (102–108) based on tryptic digestion. (B) Alanine scan at the DdrO cleavage site was conducted to assay the sequence specificity. Arg-109 and Lys-111 were also mutated to Glu. (C) The sequence specificity requirement is summarized here. Amino acid residues in red are essential to recognition and cleavage of the protease. The residues marked by black dots are variable.</p

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